Journal: International Journal of Nanomedicine

Article Title: Ginseng-berry-mediated gold and silver nanoparticle synthesis and evaluation of their in vitro antioxidant, antimicrobial, and cytotoxicity effects on human dermal fibroblast and murine melanoma skin cell lines

doi: 10.2147/IJN.S118373

Figure Lengend Snippet: Comparative study of the effect of GBAuNPs, GBE and HAuCl 4 ·3H 2 O on cell viability and on the morphology of HDF and B16 cells. Notes: Optical microscopy images ( A ) of HDF and B16 cell lines (40× magnification) at 72 h after treated with 1–100 μg/mL of GBAuNPs. Treated HDF and B16 cells with AuNPs (10 and 100 μg/mL) could be identified by dark dense clusters which are indicated with arrows; no cluster was found in control groups. Cytotoxicity effect of GBE and GBAuNPs on HDF ( B ) and B16BL6 ( C ) cell lines; GBAuNPs did not exhibit a significant cytotoxic effect on HDF and B16 cells. Cell viability was determined by MTT assay. Data are expressed as a percentage of sample-treated control and presented as mean ± SEM of three separate experiments. B16, murine melanoma B16BL6 cells. Abbreviations: AuNPs, gold nanoparticles; GBAuNPs, AuNPs from ginseng berry; GBE, ginseng berry extract; HDF, human dermal fibroblast; SEM, standard error of the mean.

Article Snippet: HDF and B16 cell lines were obtained from the Korean Cell Line Bank (Seoul, Korea).

Techniques: Microscopy, Control, MTT Assay

Journal: International Journal of Nanomedicine

Article Title: Ginseng-berry-mediated gold and silver nanoparticle synthesis and evaluation of their in vitro antioxidant, antimicrobial, and cytotoxicity effects on human dermal fibroblast and murine melanoma skin cell lines

doi: 10.2147/IJN.S118373

Figure Lengend Snippet: Comparative study of the effect of GBAgNPs, GBE and AgNO 3 on cell viability and on the morphology of HDF and B16 cells. Notes: Optical microscopy images ( A ) of HDF and B16 cell lines (40× magnification) at 72 h after treated with 1–100 μg/mL of GBAgNPs. Treated HDF and B16 cells with silver (10 μg/mL) could be identified by dark dense clusters which are indicated with arrows; no cluster was found in control groups. Cytotoxicity effect of GBE, GBAgNPs, and silver salts on HDF ( B ) and B16 ( C ) cell lines; cell viability decreased with an increase in the concentration of GBAgNPs. Cell viability was determined by MTT assay. Data are expressed as a percentage of sample-treated control and presented as mean ± SEM of three separate experiments. Abbreviations: B16, murine melanoma B16Bl6 cells; GBAgNPs, silver nanoparticles from ginseng berry; GBE, ginseng berry extract; HDF, human dermal fibroblast; MTT, 3-(4,5-dimethyl-thiazol-2yl)-2, 5-diphenyl tetrazolium bromide; SEM, standard error of the mean.

Article Snippet: HDF and B16 cell lines were obtained from the Korean Cell Line Bank (Seoul, Korea).

Techniques: Microscopy, Control, Concentration Assay, MTT Assay

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Assessment of chemotherapeutic effects on cancer cells using adhesion noise spectroscopy

doi: 10.3389/fbioe.2024.1385730

Figure Lengend Snippet: 5-Fluorouracil (5-FU) with cytotoxic and detachment-enhancing effect on the colorectal cancer cell (CRC) line HT-29 but without effect on human dermal fibroblast (HDF) cell viability and proliferation. (A) 5-FU effects via CAN spectroscopy on HT-29 cells cultivated on the CMOS MEA (analyzed at 300 kHz). Untreated HT-29 grew on the CMOS MEA and the cell-covered area rose (A-i) . After 5-FU treatment, the detection positive area declined after 72 h of cultivation (A-ii) . Statistics (A-i,A-ii) : one-way ANOVA. Statistical difference of the cell-covered area between untreated and treated HT-29 (A-iii) . Statistics (A-iii) : two-way ANOVA. (B) 5-FU effects via CAN spectroscopy on HDF cells cultivated on the CMOS MEA (analyzed at 300 kHz). Untreated and treated HDFs showed a stagnant CMOS MEA area where cells are adhered to (B-i,B-ii) . Statistics (B-i,B-ii) : one-way ANOVA. CMOS MEA area covered by HDFs (B-iii) . Statistics (B-iii) : two-way ANOVA. (C) 5-FU effects on HT-29 cells via CCK-8 assay. Untreated HT-29 cells’ optical density (OD) increased during 72 h cultivation time (C-i) , while 5-FU treated cells’ OD declined (C-ii) . (D) 5-FU effects on HDF cells via CCK-8 assay. For HDFs, the OD stays constant without (D-i) and with 5-FU treatment (D-ii) . Statistics: one-way ANOVA. (C-iii,D-iii) compared cell viability of treated cells with untreated cells as control group. Treated cells showed less cell viability for both cell types [HT-29 (C-iii) and HDF (D-iii) ]. Statistics: two-way ANOVA. (E) Cell viability from CASY recordings of treated and untreated HT-29 in the supernatant and on the chip (E-i) . Same for HDFs (E-ii) . Two-way ANOVA. Statistical significance is indicated by asterisks (n HT-29 = 4, n HDF = 4, ns, not significant. *, p ≤ 0.05. **, p ≤ 0.01. ***, p ≤ 0.001. ****, p ≤ 0.0001).

Article Snippet: The colorectal cancer (CRC) cell line HT-29 (ATCC, RRID: CVCL_0320) and the human dermal fibroblast cell line HDF (PELOBiotech GmbH, RRID: CVCL_DP66) were cultivated at 37°C in a 5% CO 2 atmosphere.

Techniques: Spectroscopy, CCK-8 Assay, Control

Journal: Advanced Healthcare Materials

Article Title: Panthenol Citrate Biomaterials Accelerate Wound Healing and Restore Tissue Integrity

doi: 10.1002/adhm.202301683

Figure Lengend Snippet: PC and PC‐PPCN promote the migration and proliferation of human dermal fibroblasts (HDF) and human epidermal keratinocytes (HEK α ) from healthy and diabetic individuals. a) Images of healthy HDF migration after exposure to saline, PC, PPCN, and PC‐PPCN for 8 h. b) Quantification of percentage wound area remaining for healthy HDF. c) Healthy HDF proliferation at 72 h. d) Images of diabetic HDF migration after exposure to saline, PC, PPCN, and PC‐PPCN for 8 h. e) Quantification of percentage of wound area remaining for diabetic HDF. f) Diabetic HDF proliferation at 72 h. g) Images of healthy HEK α migration after exposure to saline, PC, PPCN, and PC‐PPCN for 24 h. h) Quantification of percentage of wound area remaining for healthy HEK α . i) Healthy HEK α proliferation at 72 h. j) Images of diabetic HEK α migration after exposure to saline, PC, PPCN, and PC‐PPCN for 24 h. k) Quantification of percentage of wound area remaining for diabetic HEK α . l) Diabetic HEK α proliferation at 72 h ( n = 3; ns, not significant; * p < 0.05; ** p < 0.01; *** p < 0.001, **** p < 0.0001).

Article Snippet: Healthy and diabetic HEK α and HDF (Zen‐bio, passage 1–2), and HMVEC (Lonza, passage 1–2) proliferation assays were conducted by seeding cells at a density of 5 × 10 4 cells per well in 24‐well tissue culture plates and incubated overnight to allow cell attachment.

Techniques: Migration, Saline

Journal: Nature Communications

Article Title: Plasma membrane phosphatidylinositol (4,5)-bisphosphate is critical for determination of epithelial characteristics

doi: 10.1038/s41467-022-30061-9

Figure Lengend Snippet: a Immunofluorescence detection of PI(4,5)P 2 in skin sections of newborn mice. The dotted line denotes the border between the epidermis and the dermis. Results shown are representative of two independent experiments. b , c Immunofluorescence detection of PI(4,5)P 2 in epithelial (HaCaT and NMuMG) and non-epithelial (HDF, Swiss3T3, and U2OS) cell lines b . Quantification of apicolateral plasma membrane (PM) PI(4,5)P 2 signals c . In total, 138 HaCaT, 122 NMuMG, 104 HDF, 120 Swiss3T3, 102 U2OS cells were examined over two independent experiments. d – g Immunofluorescence detection of PI(4,5)P 2 in HaCaT cells treated with or without TGFβ1 for 72 h d and HaCaT cells overexpressing SNAI1 or SNAI2 f . F-actin was visualized using phalloidin. Quantification of apicolateral PM PI(4,5)P 2 signals e , g . In total, 120 control and 109 TGFβ1-treated cells were examined over two independent experiments e . In total, 100 control, 102 SNAI1-, and 115 SNAI2-expressing cells were examined over two independent experiments g . h , i PIP 2 amount in HaCaT cells, treated with or without TGFβ1 for 72 h, and HDF cells was determined using mass spectrometry h . The amount of PIP 2 with zero, one, or two double bonds and three or more double bonds in acyl chains is also shown i . N = 4 for untreated HaCaT cells, and N = 3 for TGFβ1-treated HaCaT and HDF cells. Data are represented as mean ± SD h , i . *** p = 0.0006, ** p = 0.0012 h , 0.0061 i (versus HaCaT cells) h , i . The box plots are presented with the elements: center line, median; box limits, Q1 and Q3; whiskers, 1.5× interquartile range. Outliers are also shown. Individual data points are displayed [gray points, data from the first experiments; black points, data from the second experiments c , e , g ]. Significance was tested using one-way ANOVA with Tukey-Kramer’s post hoc test c , g , h , i and the two-sided Welch’s t-test e . **** p < 0.0001 [versus HaCaT or NMuMG cells c , control cells e , g , and HaCaT cells h , i ]. Scale bar = 30 μm a or 20 μm b , d , f . Source data are provided as a Source Data file.

Article Snippet: HDF (Kurabo, Osaka, Japan), Swiss3T3 (JCRB, Osaka, Japan), U2OS (ATCC), and MG-63 (JCRB) cells were cultured in DMEM (Nissui Pharmaceutical, Tokyo, Japan) supplemented with 10% FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin.

Techniques: Immunofluorescence, Clinical Proteomics, Membrane, Control, Expressing, Mass Spectrometry

Journal: Nature Communications

Article Title: Plasma membrane phosphatidylinositol (4,5)-bisphosphate is critical for determination of epithelial characteristics

doi: 10.1038/s41467-022-30061-9

Figure Lengend Snippet: a, b Detection of cholesterol using recombinant mCherry-D4 protein (D4) in GFP-, Lyn-INPP5Ewt-GFP (INPP5E WT)-, and Lyn-INPP5Emt-GFP (INPP5E MT)-expressing HaCaT cells a . Quantification of the plasma membrane (PM) cholesterol abundance b . In total, 103 GFP-, 119 INPP5E WT-, and 104 INPP5E MT-expressing cells were examined over two independent experiments b . c Relative cholesterol levels measured using cholesterol oxidase. Data are represented as mean ± SD c . N = 3 for each group. d , e Detection of cholesterol (D4) in epithelial (HaCaT and NMuMG) and non-epithelial (HDF, Swiss3T3, and U2OS) cell lines d . Quantification of PM cholesterol signals e . In total, 92 HaCaT, 71 NMuMG, 60 HDF, 121 Swiss3T3, 84 U2OS cells were examined over two independent experiments e . f , g Detection of cholesterol (D4) in HaCaT cells treated with or without TGFβ1 for 72 h f . Quantification of PM cholesterol signals g . In total, 100 control and 110 TGFβ1-treated cells were examined over two independent experiments g . h, i Detection of cholesterol (D4), E-cadherin (E-cad), β-catenin (β-cat), claudin-1, and PARD3 in untreated (Vehicle) or U-18666A-treated HaCaT cells h . Phase images are shown (bottom panels). Images were taken from different fields of view. Quantification of E-cadherin, β-catenin, claudin-1, and PARD3 in the PM i . In total, 115 vehicle- and 121 U-18666A-treated cells were examined over two independent experiments for E-cadherin. In total, 110 vehicle- and 120 U-18666A-treated cells were examined over two independent experiments for β-catenin. In total, 115 vehicle- and 120 U-18666A-treated cells were examined over two independent experiments for claudin-1. In total, 115 vehicle- and 120 U-18666A-treated cells were examined over two independent experiments for PARD3 i . The box plots are presented with the elements: center line, median; box limits, Q1 and Q3; whiskers, 1.5× interquartile range. Outliers are also shown. Individual data points are displayed (gray points, data from the first experiments; black points, data from the second experiments b , e , g , i ). Significance was tested using one-way ANOVA with Tukey-Kramer’s post hoc test b , e and the two-sided Welch’s t-test g , i . **** p < 0.0001 (versus GFP-expressing cells b , HaCaT cells or NMuMG cells e , untreated cells g , i ). Scale bar = 30 μm ( a , d , f , h except for the bottom panels of h ) or 50 μm (bottom panels of h ). Source data are provided as a Source Data file.

Article Snippet: HDF (Kurabo, Osaka, Japan), Swiss3T3 (JCRB, Osaka, Japan), U2OS (ATCC), and MG-63 (JCRB) cells were cultured in DMEM (Nissui Pharmaceutical, Tokyo, Japan) supplemented with 10% FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin.

Techniques: Recombinant, Expressing, Clinical Proteomics, Membrane, Control